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FEBS Lett. 1997 Jun 9;409(2):207-10.

Differential involvement of caspases in apoptosis of myeloid leukemic cells induced by chemotherapy versus growth factor withdrawal.

Author information

1
Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Gent, Belgium. Willemze@rullf2.Leidenuniv.nl

Abstract

To assess the potential involvement of the caspase family in the IL-3-dependent murine myeloid leukemic cell line 32D, we studied the effect of bcl-2, crmA and three synthetic caspase inhibitors on apoptosis induced by chemotherapy or IL-3 withdrawal. Apoptosis induced by IL-3 deprivation or by ActD appears to be mediated by a crmA-insensitive pathway. Cell death by IL-3 withdrawal is inhibited by the caspase-inhibitor ZVAD-fmk, but not DEVD-fmk or YVAD-cmk. In contrast, DEVD-fmk as well as ZVAD-fmk protect 32D cells from ActD-induced apoptosis. These results indicate that different caspases are involved in apoptosis induced by growth factor withdrawal and by chemotherapy.

PMID:
9202146
DOI:
10.1016/s0014-5793(97)00507-3
[Indexed for MEDLINE]
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