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Obes Res. 1997 May;5(3):183-5.

Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation.

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Laboratory of Human Behavior and Metabolism, Rockefeller University, New York, NY 10021, USA.


Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C-->T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A-->C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.

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