Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP)

Nucleic Acids Res. 1997 Jul 1;25(13):2595-7. doi: 10.1093/nar/25.13.2595.

Abstract

The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomerase activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Primers
  • Dimerization
  • False Negative Reactions
  • Humans
  • Neoplasms / enzymology*
  • Polymerase Chain Reaction*
  • Quality Control
  • Repetitive Sequences, Nucleic Acid*
  • Telomerase / analysis*
  • Telomerase / metabolism*
  • Telomere*

Substances

  • DNA Primers
  • Telomerase