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Methods. 1997 Jun;12(2):165-71.

Purification of heat shock protein-peptide complexes for use in vaccination against cancers and intracellular pathogens.

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1
Department of Biological Sciences, Fordham University, Bronx, New York 10458, USA.

Abstract

This article describes the methods used for the purification of heat shock proteins gp96, hsp90, and hsp70 from murine and human tissues and cell lines. The heat shock proteins purified by the methods described are associated noncovalently with an array of cellular peptides. The use of heat shock protein-peptide complexes for eliciting prophylactic and therapeutic immunity to cancers and infectious diseases is discussed.

PMID:
9184380
DOI:
10.1006/meth.1997.0464
[Indexed for MEDLINE]
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