Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 1997 Jun;12(2):125-42.

Identification of genes coding for tumor antigens recognized by cytolytic T lymphocytes.

Author information

  • 1Ludwig Institute for Cancer Research, Brussels Branch, Belgium.

Abstract

Strategies have been developed to characterize tumor antigens recognized by cytolytic T lymphocytes (CTL). We use a genetic approach based on the transfection of HLA genes and cDNA libraries in COS cells to isolate the gene producing the antigenic peptide. The tumor-specific expression of this gene can be evaluated by cDNA synthesis and quantitative PCR amplification. Transfection of fragments of the isolated gene allows the identification of the region encoding the antigenic peptide. Peptides are synthesized and tested for their ability to sensitize target cells to lysis by the CTL.

PMID:
9184377
DOI:
10.1006/meth.1997.0462
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center