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Biochem J. 1997 Jun 1;324 ( Pt 2):361-4.

Activation of pro-caspase-7 by serine proteases includes a non-canonical specificity.

Author information

1
Program in Aging and Cell Death Research, The Burnham Institute, San Diego, CA 92037, USA.

Abstract

As a model to investigate the mechanism of caspase activation we have analysed the processing of pro-caspase-7 by serine proteases with varied specificities. The caspase-7 zymogen was rapidly activated by granzyme B and more slowly by subtilisin and cathepsin G, generating active enzymes with similar kinetic properties. Significantly, cathepsin G activated the zymogen by cleaving at a Gln-Ala bond, indicating that the canonical cleavage specificity at aspartic acid is not required for activation.

PMID:
9182691
PMCID:
PMC1218439
DOI:
10.1042/bj3240361
[Indexed for MEDLINE]
Free PMC Article

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