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Nat Biotechnol. 1997 Jun;15(6):553-7.

Yeast surface display for screening combinatorial polypeptide libraries.

Author information

1
Department of Chemical Engineering, University of Illinois, Urbana 61801, USA.

Abstract

Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity. C-terminal fusion to the Aga2p mating adhesion receptor of Saccharomyces cerevisiae has been used for the selection of scFv antibody fragments with threefold decreased antigen dissociation rate from a randomly mutated library. A eukaryotic host should alleviate expression biases present in bacterially propagated combinatorial libraries. Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands.

PMID:
9181578
DOI:
10.1038/nbt0697-553
[Indexed for MEDLINE]

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