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Gastroenterology. 1997 Jun;112(6):1887-94.

Early functional effects of Clostridium difficile toxin A on human colonocytes.

Author information

1
Groupe de Recherche Fonctions Secretoires des Epitheliums Digestifs, CJF INSERM 94-04, Faculte de Medecine, Nantes, France.

Abstract

BACKGROUND & AIMS:

Previous in vitro studies have shown that Clostridium difficile toxin A is able to directly affect the intestinal epithelial barrier function. The aim of this study was to examine the early effects of toxin A on mucin exocytosis and determine whether this toxin can induce the production of the chemokine interleukin 8 (IL-8) from human colonic epithelial cells.

METHODS:

Two model systems were used: the HT29-CI.16E colonic goblet cell line and primary cultures of human normal colonocytes.

RESULTS:

Toxin A exerted a rapid and dose-related inhibition of stimulated mucin exocytosis without altering baseline (constitutive) mucin exocytosis from HT29-CI.16E cells. Toxin A was also able to induce the secretion of IL-8 from both HT29-CI.16E cells and primary cultures of human normal colonocytes, as early as 2-3 hours of incubation.

CONCLUSIONS:

The results show that while toxin A is able to down-regulate stimulated mucin exocytosis, it is able to up-regulate the secretion of an important chemoattractant chemokine, IL-8. These modifications illustrate the ability of colonocytes to recruit inflammatory and immune cells that will eventually bring about major mucosal damage.

PMID:
9178681
[Indexed for MEDLINE]

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