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Immunity. 1997 May;6(5):633-42.

CD8 lineage commitment in the absence of CD8.

Author information

1
Department of Immunology, Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.

Abstract

The absence of cytotoxic T lymphocyte activity and the failure of MHC class I-restricted T cell receptor (TCR) transgenic thymocytes to mature in CD8alpha-deficient mice suggest that CD8 may be essential for CD8 lineage commitment. We report that variants of the antigenic peptide that delete TCR transgenic thymocytes from CD8 wild-type but not CD8alpha-deficient mice can restore positive selection of CD8 lineage cells in the absence of CD8. The positively selected cells down-regulate CD4, up-regulate TCR, respond to the antigenic peptide, and express CD8beta mRNA. Interestingly, there was no enhanced selection of CD4+ T cells, implying that the TCR-MHC interaction, even in the absence of CD8, provided instructive signaling for commitment to the CD8 lineage. Our results are discussed in terms of recent models of T cell lineage commitment.

PMID:
9175841
PMCID:
PMC2782926
DOI:
10.1016/s1074-7613(00)80351-9
[Indexed for MEDLINE]
Free PMC Article

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