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Scand J Gastroenterol. 1997 May;32(5):455-9.

Flagellin gene diversity among Helicobacter pylori strains and IL-8 secretion from gastric epithelial cells.

Author information

1
Dept. of Internal Medicine 6, School of Medicine, Tokai University, Bohscidai Isehara, Japan.

Abstract

BACKGROUND:

To clarify the pathological functions of the virulence factors of Helicobacter pylori, a comparative analysis was carried out on the relationship between motility, flagellar gene polymorphism, vacuolating cytotoxin (VT) production and interleukin-8 (IL-8) induction.

METHODS:

Twenty-five strains were examined for restriction fragment length polymorphism (RFLP) of the flagellin gene. Motility was measured using semisolid agar plates. Cytotoxicity was assayed using RK-13 cells. IL-8 secretion was assessed by the enzyme-linked immunosorbent assay (ELISA) methods.

RESULTS:

H. pylori was classified into four groups according to their flagellar RFLP. No differences were noted in motility or VT production among the four groups, but a significant difference was noted in IL-8 induction. In addition, highly motile strains produced more IL-8.

CONCLUSION:

This flagellar genetic polymorphism may be associated with IL-8 induction.

PMID:
9175206
DOI:
10.3109/00365529709025080
[Indexed for MEDLINE]

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