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J Reprod Med. 1997 May;42(5):260-6.

Twin zygosity. Automated determination with microsatellites.

Author information

1
Franz-Volhard Clinic, Max Delbrück Center for Molecular Medicine, Virchow Klinikum, Humboldt University, Berlin, Germany.

Abstract

OBJECTIVE:

Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; however, these methods are more than occasionally inefficient, often expensive and sometimes inaccurate. We used microsatellites as DNA markers and developed a largely automated, rapid and efficient method of determining zygosity.

STUDY DESIGN:

We used five highly polymorphic short tandem repeat loci, coamplified by polymerase chain reaction (PCR) using fluorescence-labeled primers. Thirty-six samples were simultaneously analyzed by electrophoresis and laser detection. The PCR products were sized by automated fragment analysis.

RESULTS:

We typed 132 pairs of monozygotic (MZ) and dizygotic (DZ) twins. With five markers, the probability that any twin pair was MZ if all markers were concordant was 99%.

CONCLUSION:

This method is a rapid and reliable approach to zygosity detection.

PMID:
9172114
[Indexed for MEDLINE]

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