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Biochem Biophys Res Commun. 1997 May 8;234(1):137-42.

Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis.

Author information

1
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan. snowls@tokyo.infoweb.or.jp

Abstract

A factor which inhibits osteoclast-like cell formation was found in the conditioned medium of human embryonic lung fibroblasts, IMR-90. The factor, termed osteoclastogenesis inhibitory factor, OCIF, was purified to homogeneity. OCIF is a heparin-binding basic glycoprotein and has been isolated as a monomer with an apparent molecular weight (Mr) of 60,000 and a homodimer with a Mr of 120,000. The N-terminus of OCIF is blocked and the determination of internal amino acid sequences revealed that OCIF has no homology to known proteins. OCIF inhibited in a dose-dependent manner osteoclastogenesis elicited through three distinct signaling pathways stimulated by 1 alpha,25-dihydroxy vitamin D3, parathyroid hormone, and interleukin-11, respectively, in a dose range of 1 to 40 ng/ml (IC50 = 4 to 6 ng/ml). OCIF neither inhibits bone resorption by mature osteoclasts nor exerts any other biological activities. These data strongly suggest that OCIF is a novel cytokine which specifically inhibits osteoclastogenesis.

PMID:
9168977
DOI:
10.1006/bbrc.1997.6603
[Indexed for MEDLINE]

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