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J Mol Biol. 1997 May 2;268(2):235-42.

Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli.

Author information

  • 1UPR 9073, Institut de Biologie Physico-Chimique, Paris, France.

Abstract

We have recently reported that processing occurs in the untranslated leader region of several members of a family of Gram-positive genes regulated by tRNA-mediated antitermination. We showed that cleavage at this site plays an important role in the induction of Bacillus subtilis thrS gene expression, following threonine starvation, by stabilising the downstream mRNA. Here we show that, when transferred on a plasmid, processing of the B. subtilis thrS leader can occur at the same site in Escherichia coli. Cleavage at this site is dependent on the E. coli endoribonuclease E, both in vivo and in vitro, suggesting that a functional homologue of RNase E is responsible for thrS processing in B. subtilis.

PMID:
9159466
DOI:
10.1006/jmbi.1997.0971
[PubMed - indexed for MEDLINE]
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