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J Clin Microbiol. 1997 Apr;35(4):995-8.

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.

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1
Laboratoire de Bactériologie, Université de Bordeaux 2, France. helico@labhel.u-bordeaux2.fr

Abstract

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

PMID:
9157172
PMCID:
PMC229720
[Indexed for MEDLINE]
Free PMC Article
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