Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

J Immunol Methods. 1997 Apr 25;203(2):131-9. doi: 10.1016/s0022-1759(97)00018-5.

Abstract

We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface / biosynthesis
  • Antigens, Surface / genetics*
  • Antigens, Surface / isolation & purification
  • Baculoviridae / genetics*
  • Baculoviridae / immunology
  • Cloning, Molecular / methods*
  • DNA, Complementary / isolation & purification*
  • DNA, Viral / genetics*
  • Gene Library*
  • Genetic Vectors / immunology*
  • Humans
  • Placenta / metabolism
  • Placenta / virology
  • Recombination, Genetic
  • Spodoptera / genetics
  • Spodoptera / virology

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • DNA, Complementary
  • DNA, Viral