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Biotech Histochem. 1996 Mar;71(2):84-7.

Feulgen staining of intact plant tissues for confocal microscopy.

Author information

1
Department of Environmental and Plant Biology, Ohio University, Athens, 45701-2979, USA.

Abstract

A method was developed to prepare plant structures for confocal laser scanning microscopy by combining Feulgen staining with pararosaniline and embedding in LR White(TM). This procedure preserves intact, delicate structures for three-dimensional imaging without loss from sectioning or squashing, and the slides can be viewed several times without serious photo-bleaching.

PMID:
9138536
[Indexed for MEDLINE]

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