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Clin Diagn Virol. 1996 Nov;7(2):77-84.

Typing and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction.

Author information

1
Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA. ratmar@fluctr.bcm.tmc.edu

Abstract

BACKGROUND:

Influenza virus infections are a major cause of morbidity and the identification of the type or subtype of a clinical isolate has important clinical and epidemiological implications.

OBJECTIVES:

To evaluate the ability of a reverse transcription-polymerase chain reaction (RT-PCR) assay to type and subtype clinical human isolates of influenza virus.

STUDY DESIGN:

Reference strains of influenza A H1N1, A/H3N2, and B viruses and human clinical isolates of influenza virus representing antigenic variants from the last 15 years were evaluated using an RT-PCR assay.

RESULTS:

Amplicons of 325, 198 and 365 base pairs in length were obtained from RNA extracted from influenza A/H1N1, A/H3N2 and B viruses, respectively. All human-derived A/H1N1, A/H3N2, and B reference strains and antigenic variants tested were correctly identified.

CONCLUSIONS:

RT-PCR is an effective alternative to traditional methods for typing and subtyping influenza viruses.

PMID:
9137863
DOI:
10.1016/s0928-0197(96)00254-1
[Indexed for MEDLINE]

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