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Gene. 1997 Apr 1;188(2):215-20.

Insertionally inactivated and inducible recA alleles for use in Neisseria.

Author information

1
Northwestern University Medical School, Department of Microbiology-Immunology, Chicago, IL 60611, USA. h-seifert@nwu.edu

Erratum in

  • Gene 1997 Sep 15;197(1-2):415.

Abstract

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.

PMID:
9133594
DOI:
10.1016/s0378-1119(96)00810-4
[Indexed for MEDLINE]

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