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Biochem Mol Biol Int. 1997 Apr;41(4):695-705.

Endogenous superoxide-like species and antioxidant activity in ocular tissues detected by luminol luminescence.

Author information

1
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.

Abstract

A new luminescent method was used to detect the reactive oxygen species in aqueous and vitreous humors and in homogenates of the lens and retina of laboratory rats. Superoxide-like activity per microgram protein increased in all tissues with weight of the rat, a good indicator of animal age. Superoxide dismutase, centrophenoxine, soluble vitamin E (D-alpha-Locopherol (polyethlyene glycol 1000) succinate, and N'-diphenyl-p-phenylenediamine (DPPD) reduced the luminescence. Catalase had no effect. These results are consistent with the detected species being superoxide-like.

PMID:
9111931
DOI:
10.1080/15216549700201741
[Indexed for MEDLINE]

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