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Biotechniques. 1997 Apr;22(4):752-6.

Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.

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1
CLONTECH Laboratories, Inc., Palo Alto, CA, USA.

Abstract

We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.

PMID:
9105628
[Indexed for MEDLINE]
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