The strigeoid metacercaria Diplostomulum xenopi is commonly found in the pericardial cavity of Xenopus laevis laevis. This paper provides the first description of the adult obtained from the intestine of an experimental host, the darter, Anhinga melanogaster. Natural cercarial infections were found in specimens of the freshwater snail Bulinus tropicus collected from dams in the Free State. South Africa. The life cycle was experimentally completed and all stages were described by light and scanning electron microscopy.