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Nucleic Acids Res. 1997 Mar 15;25(6):1317-8.

B lymphocyte-specific, Cre-mediated mutagenesis in mice.

Author information

1
Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany.

Abstract

Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.

PMID:
9092650
PMCID:
PMC146582
DOI:
10.1093/nar/25.6.1317
[Indexed for MEDLINE]
Free PMC Article

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