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Acta Trop. 1997 Feb;63(2-3):117-26.

Reverse transcriptase-like activity in Trypanosoma cruzi.

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Departamento de Biología Molecular, Instituto de Parasitologia y Biomedicina, Lopez Negra CSIC, Granada, Spain.


The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/ oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg+2 ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D. novobiocin, rifamycin SV and AZT.

[Indexed for MEDLINE]

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