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J Theor Biol. 1997 Feb 21;184(4):433-40.

Enzymological considerations for a theoretical description of the quantitative competitive polymerase chain reaction (QC-PCR).

Author information

1
Departamento de Biología Celular, Universidad Simón Bolivar, Caracas, Venezuela. SSCHnell@taquion.ivic.ve

Abstract

The enzymological principles of the polymerase chain reaction (PCR) and of the quantitative competitive PCR (QC-PCR) are developed, proposing a theoretical framework that will facilitate quantification in experimental methodologies. It is demonstrated that the specificity of the QC-PCR, i.e. the ratio of the target initial velocity to that of the competitor template, remains constant not only during a particular amplification but also for increasing initial competitor concentrations. Linear fitting procedures are thus recommended that will enable a quantitative estimate of the initial target concentration. Finally, expressions for the efficiency of the PCR and QC-PCR are derived that are in agreement with previous experimental inferences.

PMID:
9082073
DOI:
10.1006/jtbi.1996.0283
[Indexed for MEDLINE]

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