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Biochem Biophys Res Commun. 1997 Feb 24;231(3):523-5.

Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2.

Author information

1
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.

Abstract

To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.

PMID:
9070836
DOI:
10.1006/bbrc.1997.6089
[Indexed for MEDLINE]

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