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Biochem Biophys Res Commun. 1997 Feb 13;231(2):421-4.

Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate.

Author information

1
First Department of Internal Medicine, Nagoya University School of Medicine, Japan. j45973a@nucc.cc.nagoya-u.ac.jp

Abstract

Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1 approximately 2 microns order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 microns. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.

PMID:
9070292
DOI:
10.1006/bbrc.1997.6125
[Indexed for MEDLINE]
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