Format

Send to

Choose Destination
Biochem Biophys Res Commun. 1997 Feb 13;231(2):321-8.

PCNA and DNA polymerase delta catalytic subunit from Schizosaccharomyces pombe do not interact directly.

Author information

1
IFC1, CNRS, UPR 9044, Nillejuif, France. tratner@infobiogen.fr

Abstract

DNA polymerase delta (pol delta) is constituted of at least two subunits: the catalytic subunit of about 125 kDa (p125), and a subunit of approximately 50 kDa (p50) of unknown function. Processivity of pol delta is dependent on its auxiliary protein PCNA (proliferating cell nuclear antigen). Contradictory data were reported regarding a direct interaction between p125 and PCNA. We investigated this matter further using the baculovirus system to overexpress p125 and PCNA from S. pombe. We show that the recombinant p125 is active for basal DNA polymerase activity and for 3'-->5' exonuclease activity but is not stimulated by PCNA. Interaction between p125 and PCNA was tested by: (i) co-immunoprecipitation assay using antibodies specific for one or other polypeptides after co-expression in insect cells, and (ii) a two-hybrid assay. In both cases, no direct interaction between the two proteins was detected. Taken together, our data show that p125 and PCNA do not interact directly.

PMID:
9070271
DOI:
10.1006/bbrc.1997.6082
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center