Characterization of a type-C virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells

R G Smith; K Nooter; P Bentvelzen; M Robert-Guroff; K Harewood; M S Reitz; S A Lee; R C Gallo

doi:10.1002/ijc.2910240213

Characterization of a type-C virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells

Int J Cancer. 1979 Aug;24(2):210-7. doi: 10.1002/ijc.2910240213.

Authors

R G Smith, K Nooter, P Bentvelzen, M Robert-Guroff, K Harewood, M S Reitz, S A Lee, R C Gallo

PMID: 90662
DOI: 10.1002/ijc.2910240213

Abstract

The putative human helper virus SKA-21/A204V, isolated by Nooter et al. in 1977 from human leukemic bone-marrow cells following co-culture with normal fetal canine thymus cells, Cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. The results of these analyses show that this virus is not distinguishable from the woolly monkey type-C virus, SSAV-1, by the techniques employed.

MeSH terms

Animals
Bone Marrow / immunology
Bone Marrow / microbiology*
Cell Line
Cells, Cultured
DNA, Viral / immunology
Dogs
Electrophoresis, Polyacrylamide Gel
Humans
Leukemia / immunology
Leukemia / microbiology*
Neutralization Tests
Nucleic Acid Hybridization
RNA, Viral / immunology
RNA-Directed DNA Polymerase / metabolism
Radioimmunoassay
Retroviridae / enzymology
Retroviridae / immunology*
T-Lymphocytes / immunology*
Viral Proteins / immunology

Substances

DNA, Viral
RNA, Viral
Viral Proteins
RNA-Directed DNA Polymerase