Send to

Choose Destination
Mol Gen Genet. 1997 Feb 20;253(5):649-53.

A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein.

Author information

Département de Biochimie, Faculté des sciences et de génie, Université Laval, Québec, Canada.


An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center