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Zentralbl Bakteriol. 1997 Jan;285(2):252-7.

Epidemiological typing of Klebsiella pneumoniae by pyrolysis mass spectrometry.

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Department of Medical Microbiology, University Hospital of Wales, Cardiff, UK.


Thirteen isolates of ceftazidime-resistant Klebsiella pneumoniae from a suspected cross-infection outbreak involving patients on an intensive care unit and a haematology ward were examined in pyrolysis-mass spectrometry (Py-MS), along with eight concurrent non-outbreak-associated clinical isolates of klebsiellae as controls. Py-MS showed tight clustering of the suspected outbreak isolates, suggesting cross-infection with a single strain. Non-outbreak isolates were clearly distinct from one another and from the outbreak strain. The results confirm that Py-MS is a powerful tool for rapid strain comparison in investigations of cross-infection incidents.

[Indexed for MEDLINE]

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