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Cell. 1997 Mar 7;88(5):657-66.

Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening.

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1
Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.

Abstract

The chromosomes of lower eukaryotes have short telomeric 3' extensions. Using a primer-extension/nick-translation technique and nondenaturing hybridization, we find long 3' G-rich tails at human chromosome ends in mortal primary fibroblasts, umbilical vein endothelial cells, and leukocytes, as well as in immortalized fibroblasts. For all cells tested, >80% of the telomeres have long G-rich overhangs, averaging 130-210 bases in length, in disagreement with the conventional model for incomplete lagging-strand replication, which predicts overhangs on 50% of the chromosome ends. The observed G tails must exist during most of the cell cycle and probably result from degradation of both chromosome ends. The average lengths of the G tails are quantitatively consistent with the observed rates of human chromosome shortening.

PMID:
9054505
[Indexed for MEDLINE]
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