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J Gen Virol. 1995 Jul;76 ( Pt 7):1807-13.

The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo.

Author information

1
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.

Abstract

Processing of the 112 kDa ('112K') protein encoded by cowpea mosaic virus RNA 1 was examined in cowpea mesophyll protoplasts using a transient expression system. Cleavage of the 112K protein occurred via two alternative pathways either into VPg and 110K (24K + 87K) or into 26K (VPg + 24K) and 87K proteins. The 26K protein can be further cleaved into VPg and 24K proteins. The results support a model in which the 112K protein functions as the precursor of VPg during initiation of replication.

PMID:
9049386
DOI:
10.1099/0022-1317-76-7-1807
[Indexed for MEDLINE]

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