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J Immunol Methods. 1997 Feb 14;201(1):67-75.

High-level secretion of two antibody single chain Fv fragments by Pichia pastoris.

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Department of Laboratory Medicine and Pathology, School of Medicine, University of Minnesota, Minneapolis 55455, USA.


The diagnostic and therapeutic applications of antibody single-chain Fv (sFv) fragments often require large amounts of protein that can be problematic and expensive to obtain. Here we report the secretion of two sFv fragments by the yeast Pichia pastoris at levels up to 250 mg/l. Soluble sFv fragments were purified from culture supernatants in one step by affinity or metal-chelating chromatography, and were indistinguishable from their bacterially expressed counterparts in terms of affinity. Secretion of functional sFv fragments by Pichia pastoris provides a low cost, high yield alternative to current sFv expression systems.

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