Send to

Choose Destination
Microbiology. 1997 Jan;143 ( Pt 1):197-202.

Use of morphology index histograms to quantify populations of the fungal pathogen Paracoccidioides brasiliensis.

Author information

Laboratories of Mycology and Structural Biology, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.


To quantify the dimorphic process in wild and mutant strains of Paracoccidioides brasiliensis, we defined a morphology index (Mi) in terms of the maximum cell length (l), maximum cell diameter (d), and septal diameter (s), according to the equation Mi = 2.13 + 1.13 log10 (ls/d2), whose intercept and slope were such that Mi was around 1 for yeast (spherical) cells or 4 for hyphal (elongated) cells. This discriminatory power was used to quantify morphological population mixtures through Mi histograms. During the temperature-induced dimorphic transition (either way), mean Mi (Mi) varied linearly with time, suggesting a continuity in the process. Also, in wild strains and mutants thereof we found an inverse relationship between Mi and content of both cell wall chitin and 1,3-alpha-glucan.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center