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Mol Cell Probes. 1996 Dec;10(6):471-5.

Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR.

Author information

1
Department of Microbiology, Technical University of Gdańsk, Poland.

Abstract

The presence or absence of the mecA gene, the determinant of resistance to all beta-lactam antibiotics, was examined in clinical isolates of Staphylococcus aureus by multiplex polymerase chain reaction (MPCR). Two pairs of primers were used, which yielded two specific products; a 280-bp nuc- based PCR fragment (amplification product of the nuc gene encoding specific Staphylococcus aureus nuclease) and a 533-bp mecA-based PCR fragment (amplification product of the mecA gene). The MPCR system was designed to be incorporated into the work flow in clinical diagnostic laboratories as a routine analysis.

PMID:
9025087
DOI:
10.1006/mcpr.1996.0066
[Indexed for MEDLINE]

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