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Fertil Steril. 1997 Feb;67(2):355-61.

Autoimmunogenicity of the human sperm protein Sp17 in vasectomized men and identification of linear B cell epitopes.

Author information

1
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599, USA.

Abstract

OBJECTIVE:

To assess whether human sera positive for antisperm antibodies have detectable levels of Sp17 autoantibodies and to determine the linear B cell epitopes to which these are raised for both native and recombinant Sp17.

DESIGN:

Enzyme-linked immunoaborbent assays were performed against recombinant HSp17 on 15 serum samples from prevasovasostomy and postvasovasostomy patients. Positive sera then were used in mimotope analyses to determine HSp17 immunodominant linear B cell epitopes. These were compared with the linear B cell epitopes of recombinant HSp17.

SETTING:

University research laboratory.

PATIENT(S):

Fifteen vasectomized or vasovasostomized men.

MAIN OUTCOME MEASURE(S):

Serum antibody reactivity to human Sp17.

RESULT(S):

Sera from vasectomized and vasovasostomized men exhibit Sp17 antibodies raised predominantly to two immunodominant linear B cell epitopes (amino acids 4 to 19 and amino acids 118 to 127), which differed from those of recombinant HSp17 (amino acids 52 to 79 and amino acids 124 to 136).

CONCLUSION(S):

The results show that Sp17 is an antigen to which vasectomized men raise autoantibodies. Two linear B cell epitopes predominate in native Sp17 and these differ from (but overlap with) those of the bacterially expressed recombinant protein.

PMID:
9022615
DOI:
10.1016/S0015-0282(97)81923-1
[Indexed for MEDLINE]

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