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Gene. 1997 Jan 3;184(1):33-7.

Molecular analysis of a Penicillium chrysogenum GATA factor encoding gene (sreP) exhibiting significant homology to the Ustilago maydis urbs1 gene.

Author information

1
Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria. hubertus.haas@uibk.ac.at

Abstract

Employing a PCR-aided strategy, a Penicillium chrysogenum gene (sreP) encoding a putative GATA-transcription factor has been cloned and characterized. Comparison of the genomic and cDNA sequences revealed the presence of an open reading frame (ORF) encoding a protein of 532 amino acids (aa) which is interrupted by two introns. The deduced aa sequence of sreP reveals 50% identity to a regulator of siderophore biosynthesis (URBS1) from Ustilago maydis over a stretch of 200 aa containing two GATA-type zinc finger motifs and a Cys-rich intervening sequence. Northern blot analysis indicated two transcripts of 2.2 and 2.7 kb in approximately equivalent amount. due to two major transcription start sites.

PMID:
9016950
DOI:
10.1016/s0378-1119(96)00570-7
[Indexed for MEDLINE]

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