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Nucleic Acids Res. 1997 Feb 1;25(3):675-6.

An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.

Author information

1
Department of Medical Microbiology, St George's Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK.

Abstract

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.

PMID:
9016612
PMCID:
PMC146465
DOI:
10.1093/nar/25.3.675
[Indexed for MEDLINE]
Free PMC Article

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