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FEBS Lett. 1997 Jan 20;401(2-3):148-52.

Ceramide induces apoptosis in PC12 cells.

Author information

1
School of Biological Sciences, Flinders University of South Australia, Adelaide. P.Hartfield@flinders.edu.au

Abstract

The novel lipid second messenger, ceramide, induced apoptosis in PC12 cells as determined morphologically by nuclear appearance and internucleosomal DNA fragmentation. Apoptosis was induced by exogenous C2-ceramide in a dose- and time-dependent manner. Natural ceramide and C6-ceramide had a similar effect. This response was specific since the structural analog C2-dihydroceramide and other related lipids failed to initiate apoptosis. The apoptotic effect of ceramide also depends critically on cell plating density. Furthermore, the peptide inhibitor of interleukin-1beta converting enzyme (ICE)-like proteases, Z-VAD.FMK, completely prevented the nuclear changes induced by ceramide, implicating the involvement of ICE-like protease activation in ceramide-induced apoptosis in PC12 cells.

PMID:
9013876
DOI:
10.1016/s0014-5793(96)01460-3
[Indexed for MEDLINE]
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