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Cell. 1997 Jan 24;88(2):205-11.

Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok.

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1
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Abstract

A 62 kDa protein is highly phosphorylated in many cells containing activated tyrosine kinases. This protein, characterized mainly by its avid association with rasGAP, has proved elusive. Anti-phosphotyrosine antibody was used to purify p62. From peptide sequence, molecular cloning revealed a cDNA encoding a novel protein, p62dok, with little homology to others but with a prominent set of tyrosines and nearby sequences suggestive of SH2 binding sites. In cells, v-Abl tyrosine kinase binds and strongly phosphorylates p62dok, which then binds rasGAP. A monoclonal antibody, 2C4, to the rasGAP-associated p62 reacts with p62dok. Thus, p62dok appears to be the long-sought major substrate of many tyrosine kinases.

PMID:
9008161
DOI:
10.1016/s0092-8674(00)81841-3
[Indexed for MEDLINE]
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