Send to

Choose Destination
J Neurosci Methods. 1996 Dec 28;70(2):201-10.

Aspects of the quantitative analysis of neurons in the cerebral cortex.

Author information

Institute of Anatomy and Cell Biology, University of Göteborg, Sweden.


We address three problems concerning the quantitative analysis of nerve cell distribution in the cerebral cortex: (i) preparatory tissue deformation (shrinkage); (ii) difficulties in differentiating between small neurons and astroglia; and (iii) the bias introduced by the counting method. We found that staining with Richardson's solution led to no shrinkage in Vibratome-cut sections of aldehyde-fixed rat brains, but did result in staining of the neurons and left the glial cells unstained. This was in striking contrast to Nissl staining which introduced a linear shrinkage of 20-30% and stained all kinds of cortical cells indiscriminately. A computer-based unbiased counting method was implemented by taking advantage of the stereological procedure referred to as the 'optical disector' (Gundersen, H.J.G. (1986) Stereology of arbitrary particles, J. Microsc., 143: 3-45).

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center