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J Neurosci Methods. 1996 Dec 28;70(2):201-10.

Aspects of the quantitative analysis of neurons in the cerebral cortex.

Author information

1
Institute of Anatomy and Cell Biology, University of Göteborg, Sweden. skoglund@mednet.gu.se

Abstract

We address three problems concerning the quantitative analysis of nerve cell distribution in the cerebral cortex: (i) preparatory tissue deformation (shrinkage); (ii) difficulties in differentiating between small neurons and astroglia; and (iii) the bias introduced by the counting method. We found that staining with Richardson's solution led to no shrinkage in Vibratome-cut sections of aldehyde-fixed rat brains, but did result in staining of the neurons and left the glial cells unstained. This was in striking contrast to Nissl staining which introduced a linear shrinkage of 20-30% and stained all kinds of cortical cells indiscriminately. A computer-based unbiased counting method was implemented by taking advantage of the stereological procedure referred to as the 'optical disector' (Gundersen, H.J.G. (1986) Stereology of arbitrary particles, J. Microsc., 143: 3-45).

PMID:
9007760
DOI:
10.1016/s0165-0270(96)00119-7
[Indexed for MEDLINE]

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