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Curr Microbiol. 1997 Feb;34(2):118-21.

Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus.

Author information

1
The International Center of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya.

Abstract

A local isolate of Bacillus thuringiensis,designated L1-2, that is toxic to Chilo partellus was found to be toxic to the adult tsetse fly, Glossina morsitans morsitans. The delta-endotoxin crystals derived from the isolate gave a major protein band with a molecular weight of Mr 130,000-140,000 on denaturing polyacrylamide gel electrophoresis. The sequence of the cloned gene was found to be similar to that of the B. thuringiensis subsp. kurstaki HD-73 cryIA(c) gene, having one amino acid difference at position 148 and four additional DNA differences.

PMID:
9003589
DOI:
10.1007/s002849900154
[Indexed for MEDLINE]

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