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Mol Gen Genet. 1996 Dec 13;253(3):342-52.

Isolation of a carbon source-regulated gene from Ustilago maydis.

Author information

1
Institut für Genetik und Mikrobiologie, Universität München, Germany.

Abstract

We have isolated a carbon source-regulated gene from the phytopathogenic fungus Ustilago maydis by use of a promoter-probe vector. This gene, called crg1, is strongly induced by L-arabinose and efficiently repressed by D-glucose and D-xylose. The predicted 36.5-kDa mature crg1 gene product lacks similarity to known proteins but is likely to be secreted. Sequences required for regulated expression of a reporter gene are contained within a 3.6-kb fragment upstream of the crg1 gene. The promoter of crg1 fulfils requirements for an efficient controllable gene expression system in U. maydis.

PMID:
9003321
DOI:
10.1007/pl00008601
[Indexed for MEDLINE]

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