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Gene. 1996 Dec 12;183(1-2):35-40.

Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi.

Author information

1
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USA.

Abstract

The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

PMID:
8996084
DOI:
10.1016/s0378-1119(96)00417-9
[Indexed for MEDLINE]

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