Abstract
To investigate the effects of PACAP-27 on electrolyte transport across the isolated human intestinal mucosa, changes in short-circuit current (Isc) were measured in Ussing chamber experiments. Serosally added PACAP-27 increased Isc in a concentration-dependent manner, eliciting a similar maximal effect in both the jejunal and the colonic mucosa. Bumetanide inhibited Isc responses, indicating stimulation of Cl- secretion. The potency and efficacy of PACAP-27 were comparable to those of VIP, suggesting that both peptides activate intestinal secretion by way of a common receptor located in the basolateral membrane of the intestinal epithelium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Colon / physiology
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Colon / physiopathology
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Crohn Disease / physiopathology*
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Dose-Response Relationship, Drug
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Electrolytes / metabolism*
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Gastrointestinal Hormones*
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Humans
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In Vitro Techniques
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Intestinal Mucosa / drug effects
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Intestinal Mucosa / physiology*
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Intestinal Mucosa / physiopathology
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Jejunum / physiology
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Membrane Potentials / drug effects
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Natriuretic Peptides
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Neuropeptides / pharmacology*
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Neurotransmitter Agents / pharmacology
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Peptides / pharmacology
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Tetrodotoxin / pharmacology
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Vasoactive Intestinal Peptide / pharmacology
Substances
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ADCYAP1 protein, human
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Electrolytes
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Gastrointestinal Hormones
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Natriuretic Peptides
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Neuropeptides
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Neurotransmitter Agents
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Peptides
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Pituitary Adenylate Cyclase-Activating Polypeptide
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guanylin
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Vasoactive Intestinal Peptide
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Tetrodotoxin