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Arch Biochem Biophys. 1997 Jan 1;337(1):115-20.

Lucigenin (bis-N-methylacridinium) as a mediator of superoxide anion production.

Author information

1
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.

Abstract

Lucigenin (bis-N-methylacridinium) (Luc2+) has frequently been used for the luminescent detection of O2-. In fact, the pathway leading to this luminescence requires univalent reduction of Luc2+ to LucH.+ followed by formation of an unstable dioxetane by reaction of LucH.+ with O2-. It is now shown that LucH.+ rapidly autooxidizes and so produces O2-. Luc2+ can thus mediate O2- production in systems, such as glucose plus glucose oxidase, in which there is ordinarily no O2- production. Luc2+ luminescence can thus be used as the basis for assaying superoxide dismutase activity but should not be used for measuring, or even detecting, O2-.

PMID:
8990275
DOI:
10.1006/abbi.1997.9766
[Indexed for MEDLINE]

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