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Vet Immunol Immunopathol. 1996 Nov;54(1-4):197-204.

Restricted B-cell responses to microbial challenge of the respiratory tract.

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CSL Ltd. A.C.N. 051 588 348, Parkville Victoria, Australia.


The local B-cell response in the respiratory tract to infectious challenge has been analyzed in pigs and calves using two techniques: flow cytometry and antibody secreting cell (ASC) probes. Pneumonia in pigs caused by experimental infection with Mycoplasma hyopneumoniae resulted in a 25-fold increase in the B-cell population in BAL and lung parenchyma 28 days post infection. ASC probes revealed that the B-cell response of immune pigs to a large challenge infection was localized to lung parenchyma and tracheobronchal lymph nodes. Naive calves infected with Pasteurella multocida had a 5-fold increase in the B-cell blast population in lung parenchyma and BAL, and a greater than 60-fold increase in the draining lymph node at 9 days post infection. The ASC probes prepared post challenge from immune calves showed the response to be localized to the draining lymph nodes, with little response in lung parenchyma. A major finding was that ASC probes prepared from lung parenchyma and from pulmonary lymph nodes of both calves and pigs recognized a restricted range of bacterial antigens, particularly compared to the range of antigens recognized by concurrently circulating sera. The use of ASC probes demonstrates that there is a restricted B-cell repertoire in the respiratory tract.

[Indexed for MEDLINE]

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