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Trends Biotechnol. 1996 Dec;14(12):465-70.

Biophysical methods for the determination of antibody-antigen affinities.

Author information

1
Institute of Molecular Biology and Biophysics, ETH Hoenggerberg, Z├╝rich, Switzerland. dario@mol.biol.ethz.ch

Abstract

Binding specificities against virtually any antigen can be isolated from antibody libraries displayed on filamentous phage. The determination of antibody-antigen affinity constants and binding kinetics is an important part of antibody characterization, and may be predictive of antibody performance in biotechnological applications. This article, intended as a guideline for the scientist who isolates a novel antibody and wishes to characterize its binding properties, presents the authors' view on widely used methodologies for the quantitative determination of antibody-antigen interactions.

PMID:
8987915
DOI:
10.1016/S0167-7799(96)10067-6
[Indexed for MEDLINE]
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