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FEBS Lett. 1996 Dec 9;399(1-2):163-5.

Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease alpha-sarcin.

Author information

1
Instituto de Estructura de la Materia, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Abstract

alpha-Sarcin is a ribosome-inactivating protein which selectively cleaves a single phosphodiester bond in a universally conserved sequence of the major rRNA. The solution structure of a-sarcin has been determined on the basis of 1898 distance and angular experimental constraints from NMR spectroscopy. It reveals a catalytic mechanism analogous to that of the T1 family of ribonucleases while its exquisite specificity resides in the contacts provided by its distinctive loops.

PMID:
8980143
DOI:
10.1016/s0014-5793(96)01320-8
[Indexed for MEDLINE]
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