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Cell. 1996 Dec 13;87(6):1123-34.

Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase.

Author information

1
Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, New Jersey 08855, USA.

Abstract

At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase. We show that transcription activation at Class II CAP-dependent promoters requires not only the previously characterized interaction between an activating region of CAP and the RNA polymerase alpha subunit C-terminal domain, but also an interaction between a second, promoter-class-specific activating region of CAP and the RNA polymerase alpha subunit N-terminal domain. We further show that the two interactions affect different steps in transcription initiation. Transcription activation at Class II CAP-dependent promoters provides a paradigm for understanding how an activator can make multiple interactions with the transcription machinery, each interaction being responsible for a specific mechanistic consequence.

PMID:
8978616
PMCID:
PMC4430116
[Indexed for MEDLINE]
Free PMC Article
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